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Proteintech p erk
P Erk, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 612 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+erk1+2/Phospho-ERK1%2F2+(Thr202%2FTyr204)+Antibody/10__1002_slash_bmm2__70108-287-44-64
Average 96 stars, based on 612 article reviews
p erk - by Bioz Stars, 2026-10
96/100 stars

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Immunoprecipitation:

Article Title: The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs
Article Snippet: Chromatin was isolated and sheared to an average size of 200–500 bp under the condition (power, high intensity; cycle, 30 s on/30 s off for 30 cycles; temperature, 4 °C) using a Bioruptor Pico Sonication Device (cat. no. B01080010, Hologic Diagenode, Belgium). .. Immunoprecipitation was carried out overnight at 4 °C using 1 μg IP grade antibodies against p-CREB1 (cat. no. 81871-1-RR, Proteintech), p-ERK1/2 (Thr202/Tyr204), p-STAT3 (Ser727) (same with Western blot), and TCF4 (cat. no. 22337-1-AP, Proteintech). .. Next day, to the immune complex were added 30 μL Pierce Protein A/G magnetic beads (cat. no. 88802, Thermo Fisher Scientific) slurry after vortxeing, and washed, eluted, and reverse cross-linked, followed by DNA purification using a VAHTS Universal Plus DNA Library Prep Kit V4 (cat. no. ND801-01, Vazyme).

Western Blot:

Article Title: The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs
Article Snippet: Chromatin was isolated and sheared to an average size of 200–500 bp under the condition (power, high intensity; cycle, 30 s on/30 s off for 30 cycles; temperature, 4 °C) using a Bioruptor Pico Sonication Device (cat. no. B01080010, Hologic Diagenode, Belgium). .. Immunoprecipitation was carried out overnight at 4 °C using 1 μg IP grade antibodies against p-CREB1 (cat. no. 81871-1-RR, Proteintech), p-ERK1/2 (Thr202/Tyr204), p-STAT3 (Ser727) (same with Western blot), and TCF4 (cat. no. 22337-1-AP, Proteintech). .. Next day, to the immune complex were added 30 μL Pierce Protein A/G magnetic beads (cat. no. 88802, Thermo Fisher Scientific) slurry after vortxeing, and washed, eluted, and reverse cross-linked, followed by DNA purification using a VAHTS Universal Plus DNA Library Prep Kit V4 (cat. no. ND801-01, Vazyme).

Membrane:

Article Title: Recombinant Filaggrin-2 microneedles reverse androgenetic alopecia by rescuing mitochondrial dysfunction in dermal papilla cells.
Article Snippet: Androgenetic alopecia (AGA) is characterized by dihydrotestosterone (DHT)-induced damage to dermal papilla cells (DPCs), leading to hair follicle miniaturization.. Current therapies (minoxidil, finasteride) are limited by single-target mechanisms, poor skin penetration, and systemic side effects, highlighting an urgent need for innovative treatments.. In this study, we identified DHT-mediated downregulation of 1 These authors contributed equally.

Incubation:

Article Title: Recombinant Filaggrin-2 microneedles reverse androgenetic alopecia by rescuing mitochondrial dysfunction in dermal papilla cells.
Article Snippet: Androgenetic alopecia (AGA) is characterized by dihydrotestosterone (DHT)-induced damage to dermal papilla cells (DPCs), leading to hair follicle miniaturization.. Current therapies (minoxidil, finasteride) are limited by single-target mechanisms, poor skin penetration, and systemic side effects, highlighting an urgent need for innovative treatments.. In this study, we identified DHT-mediated downregulation of 1 These authors contributed equally.

Recombinant:

Article Title: Recombinant Filaggrin-2 microneedles reverse androgenetic alopecia by rescuing mitochondrial dysfunction in dermal papilla cells.
Article Snippet: Androgenetic alopecia (AGA) is characterized by dihydrotestosterone (DHT)-induced damage to dermal papilla cells (DPCs), leading to hair follicle miniaturization.. Current therapies (minoxidil, finasteride) are limited by single-target mechanisms, poor skin penetration, and systemic side effects, highlighting an urgent need for innovative treatments.. In this study, we identified DHT-mediated downregulation of 1 These authors contributed equally.

Labeling:

Article Title: Self-Assembled Rg3/Naringenin Nanoparticles for Targeted Brain Delivery: A Promising Therapeutic Approach for Early Alzheimer’s Disease
Article Snippet: The ROS detection kit was purchased from Beyotime Biotechnology Co. Ltd. (Shanghai, China) and the JC-1 detection kit was purchased from Beyotime Biotechnology Co. Ltd. (Shanghai, China). .. Primary antibody against ZO-1, Claudin-5, Occludin, p-ERK1/2, ERK1/2, c-Fos, Aβ and tau were all purchased from Proteintech Group, Wuhan, China and we labeled the horseradish peroxidase of the 2nd antibody as (PI Proteintech Group, Wuhan, China). ..

Article Title: Self-Assembled Rg3/Naringenin Nanoparticles for Targeted Brain Delivery: A Promising Therapeutic Approach for Early Alzheimer’s Disease
Article Snippet: The ROS detection kit was purchased from Beyotime Biotechnology Co. Ltd. (Shanghai, China) and the JC-1 detection kit was purchased from Beyotime Biotechnology Co. Ltd. (Shanghai, China). .. Primary antibody against ZO-1, Claudin-5, Occludin, p-ERK1/2, ERK1/2, c-Fos, Aβ and tau were all purchased from Proteintech Group, Wuhan, China and we labeled the horseradish peroxidase of the 2nd antibody as (PI Proteintech Group, Wuhan, China). ..

other:

Article Title: The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs.
Article Snippet: Chromatin was isolated and sheared to an average size of 200–500 bp under the condition (power, high intensity; cycle, 30 s on/30 s off for 30 cycles; temperature, 4 °C) using a Bioruptor Pico Sonication Device (cat. no. B01080010, Hologic Diagenode, Belgium).



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